THE EFFECTS OF AMPICILLIN ON THE GUT MICROBIOME OF LABORATORY MICE, MUS MUSCULUS.

THE EFFECTS OF AMPICILLIN ON THE GUT MICROBIOME OF LABORATORY MICE, MUS MUSCULUS.

Ampicillin is a commonly prescribed antibiotic used to treat bacterial infections and was developed to overcome drug resistance. However, it may also impact healthy gut bacteria. Understanding the effects of Ampicillin on the gut microbiome is important for maintaining healthy gut function. To determine the impact of Ampicillin on the mouse gut microbiome, fecal samples from two mice were subjected to 16S Metagenomic Sequencing before and after treatment. Treatment involved administering Ampicillin for seven days orally through the water. DNA was extracted using the QIAamp PowerFecal Pro DNA Kit by Qiagen. 16S Metagenomic Sequencing was completed by Omega Bioservices, and the difference in the composition of the gut microbiome bacteria before and after Ampicillin was analyzed. It was expected that Ampicillin would adversely affect the composition of the gut microbiome by affecting bacterial diversity. When bacterial diversity is disrupted, it could allow opportunistic pathogens to increase in number, which can be observed in Metagenomic Sequencing.

Destiny Martin

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Repository Submission Date: 5/1/2023 10:31:00 PM
Poster Presentation Date: 5/4/2023

Amanda Rollins, PhD. (Biology - Wilmington College)

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